FORMULATION AND EVALUATION OF PROBIOTIC AND PREBIOTIC LOADED PELLETS BY EXTRUSION AND SPHERONIZATION FOR IMPROVED STORAGE VIABILITY

Objective: The present study aims to prepare stains-loaded enteric-coated pellets by extrusion and spheronization technique for acidic environment protection improve the viability of strains during storage. Methods: Lactobacillus casei plantarum are proven have various therapeutic prophylactic uses in human beings, but low stability storage transit site action has limited their action. Pellets were prepared incorporating probiotic D1-D9 (L. casei) E1-E9 plantarum) further enteric-coating pellets, which evaluated particle size, loss on drying, friability, micromeritic properties, viability, disintegration, survivability bile juices, studies 90 d respectively. Results: method employed preparing showed good % yields with a rage 1400-850 µm. LoD values range 3.07±0.30% 2.13±0.11%; all flow properties friability an acceptable range. SEM images revealed that had smooth uniformly surfaces. results ranged from 8.78±0.31 8.53±0.15 log CFU/g 8.47±0.15 8.85±0.22 both L. enteric coated Disintegration time was<15 min formulations. provided adequate against environment. Studies simulated gastrointestinal conditions demonstrated formulations D7 E7 higher among at end 3 h. concentration Inulin pectin combination proved better formulation study. Conclusion: This suggested using techniques can be prebiotics (Inulin Pectin) utilized formulate dosage forms improved physiological real-time